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in vitro proteome-assisted MRM for Protein Absolute QuanTification
[Nonlabeled-TTOF5600]
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Nonlabeled-TTOF5600
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Keyword
GeneID
KEGG pathway
Reactome Event
GO [ Molecular Function ]
GO [ Biological Process ]
GO [ Cellular Component ]
Query
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Label
heavy
heavy and light
Max. peptide num.
Min. peptide
length
Max. fragment num.
MS overlap range
MS range
-
Class
Class I
Class II
Class III
Marker
Input RTM* profile(min.)
17.8
19.6
22.5
25.3
28
29.4
31.4
31.5
33.2
33.8
35.4
38
example
*MRMplus Retention
Time Marker (Funakoshi)
Charge
1+
2+
3+
4+
Low priority peptides
Miss cleaved
Multiple hits per single protein
« containing these amino acids »
Methionine
N_term Q or E
« containing these modifications »
Gln->pyro-Glu
Oxidation (M)
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