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Label heavy    haevy and light Max. peptide num. Min. peptide
length
Max. fragment num. MS overlap range MS range -
Class Class I Class II Class III Marker Input RTM* profile(min.) 17.8
19.6
22.5
25.3
28
29.4
31.4
31.5
33.2
33.8
35.4
38
example
*MRMplus Retention
Time Marker (Funakoshi)
Charge 1+  2+  3+  4+ 
Low priority peptides Miss cleaved  Multiple hits per single protein
« containing these amino acids »
Methionine  Tyrosine N_term Q or E 
« containing these modifications »
Gln->pyro-Glu  Oxidation (M)
mTRAQ:13C(3)15N(1) (Y)

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